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rocG [2017-02-27 15:02:04]
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rocG [2017-02-27 15:02:04]

trigger enzyme: catabolic glutamate dehydrogenase induced by arginine, ornithine or proline, subject to carbon catabolite repression
locus
BSU37790
pI
6.00
mw
46.00 kDa
protein length
424 aa Sequence Blast
gene length
1272 bp Sequence Blast
function
arginine utilization, controls the activity of GltC
product
trigger enzyme: glutamate dehydrogenase (major)
essential
no
ec
1.4.1.2
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.4|Utilization of arginine/ ornithine]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.2|Trigger enzymes that control gene expression by protein-protein interaction with transcription factors]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|AhrC regulon], [SW|CcpA regulon], [SW|RocR regulon], [SW|SigL regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,880,740 -> 3,882,014

    Phenotypes of a mutant

  • Poor growth on complex media such as SP (sporulation medium). No growth in minimal media with arginine as the only carbon source. Rapid accumulation of suppressor mutants ([gene|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|''gudB1'']]])
  • sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin (suppressed by activation of ''[gene|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|gudB]'') due to the downregulation of the [SW|SigW regulon] [Pubmed|22178969]
  • transcription profile of a ''[gene|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG] [gene|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|gudB]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&submit.x=22&submit.y=9 GEO] [Pubmed|22178969]

    • The protein

    Catalyzed reaction/ biological activity

  • L-glutamate H2O NAD = 2-oxoglutarate NH3 NADH H (according to Swiss-Prot), controls the activity of the [protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC] transcription activator [Pubmed|17608797]

    • Protein family

  • Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|GudB]

    • Kinetic information

  • KM [glutamate] = 2.9 mM, KM [ammonium] = 18 mM [Pubmed|20630473]

    • [SW|Cofactors]

  • NAD/NADH H

    • Structure

  • [PDB|3K92] (super-repressor mutant that is capable of constitutive inactivation of [protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC], E93K mutation) [Pubmed|20630473]
    • Expression and Regulation
    [[this]]

    Biological materials

    Mutant

  • GP747 (spc), GP726 (aphA3), GP810 (del tet), GP1157 (cat) all available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • expression of native ''rocG'' in ''B. subtilis'': pGP529 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab [Pubmed|18326565]
  • for purification of RocG from ''E. coli'' carrying an N-terminal Strep-tag: pGP902 (in [SW|pGP172]), a series of ''rocG'' variants is also available in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pWH844]: pGP860, available in [SW|Jörg Stülke]'s lab
  • purification from ''B. subtilis'' with an N-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP380]): pGP1709, available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Jörg Stülke]'s lab [Pubmed|17183217]

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [SW|Fabian Commichau] University of Göttingen, Germany
  • [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

    • References

    Reviews

  • 19698086,8299344,7705101,19895831,22625175

    • Enzymatic activity of RocG

  • 18603778,16244435,16195607,18326565,9829940,20630473,21965396,25711804

    • Function in the control of [protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC] activity

  • 15150225,17994626,17608797,17183217,20630473

    • Expression of ''rocG

  • 12634342,15150224,10468601,9829940,22900538,20817675,25777015,25880922,27766092

    • Structural analysis of glutamate dehydrogenase

  • 8263917,20630473

    • Bypass of ''rocG'' mutations

  • 21219666,22178973,23785476

    • Additional publications

  • 23338837,23419162,22178969,24473333